The estimated eight-metre long snake was spotted on Thursday under a fallen tree on the island of Penang.
It died yesterday after giving birth, Herme Herisyam, operations chief for Penang’s Civil Defence Department’s southwest district.
The current record for longest snake ever caught is 7.67m, Guinness World Records says.
Medusa, a reticulated python weighing 158kg (24st 12lb), is kept at a haunted house in Kansas City in the US state of Missouri.
The Malaysian snake, also thought to be a reticulated python, has not been officially measured by record keepers.
Herisyan said it weighed 250kg and took 30 minutes to rescue, the BBC reported.
It was due to be transferred to the government’s Department of Wildlife before it died.